Figure 3From: Cytokines and olfactory bulb microglia in response to bacterial challenge in the compromised primary olfactory pathway (A) Line drawing of transverse section of olfactory bulbs and mucosa shows the locations from which micrographs (B-E) were obtained. At 6 h post-inoculation, FITC-conjugated bacteria (arrows) are present in the olfactory epithelium (OE) and olfactory nerve bundles (NB) in the lamina propria (LP; B) and in the olfactory nerve layer (ONL) and glomerular layer (GmL) of the olfactory bulb (C). Inset in c shows aggregates of bacteria. At 24 h post-inoculation, there appear to be more FITC-conjugated bacteria in the ONL and GmL of the treated side with the corresponding layers of the untreated side (ONL/NT, GmL/NT) showing the presence of a small number of bacteria (D). Numerous FITC-conjugated S. aureus were located near the nares (E). At 5 days post-inoculation, FITC-conjugated bacteria are restricted largely to the ONL (F). NC = nasal cavity; EPL = external plexiform layer. Scale bar = 200 μm (B,C,D,F), 500 μm (E), 100 μm (inset in C).Back to article page