Figure 4

CNS-targeting Tregs can reduce EAE symptoms. (A) Ten mice in three groups were given 1 × 10⁵ CAR Tregs, CD4⁺ Mock T cells or PBS alone by i.n. administration at the peak of EAE inflammation (15 days post-EAE immunization) and thereafter were monitored for EAE symptoms. Ten days post cell treatment all EAE mice in the CAR Treg group were cured (P < 0.001). At end point (15 days post cell treatment) four out of ten EAE mice in the Mock-treated group still exhibited EAE symptoms. The experiment was repeated three times with similar results. (B) Symptom-free mice from each treatment group were given a second dose of EAE-inducing inoculum and monitored for EAE symptoms. CAR Treg-treated mice were able to resist EAE inflammation to a higher extent than CD4+ Mock-treated mice (P < 0.001). Pooled data from six EAE mice (three/group from two separate experiments) are shown in the figure. Scores for individual mice are shown separately in the figure. (C) Ten EAE mice in three groups were administered 1x10⁵ CAR Tregs, CD4⁺ Mock T cells or PBS alone by i.p. injection at peak of EAE inflammation (day 15). At end point (day 15 post-treatment) all mice in the CAR Τreg group were cured but six out of ten mice in the Mock CD4⁺ T cell group still exhibited EAE symptoms. Statistics are analyzed with Mann-Whitney test using GraphPad prism software. *P < 0.05, **P < 0.01, ***P < 0.001.