Effects of SAHA on I-TAC production and ICAM-1 expression by IFN-γ-activated human astrocytes. Human astrocytes were pretreated with or without SAHA at the concentrations indicated for 1 h before stimulation with IFN-γ (50 U/ml). Astrocytes in the control group were incubated with medium only. After 48 h incubation, I-TAC concentrations were determined in the cell-free supernatants of astrocytes (A) and the astrocytic expression of ICAM-1 was measured (B). *Significantly different from IFN-γ stimulation only. #Significantly different from unstimulated control. n = 7. O.D., optical density units.