Table 1 Amoeboid microglia isolation procedure

1.

Dissection and mechanical dissociation of cerebral tissue from newborn rat

1 hour

2.

Incubate in flask with defined medium, 37 °C

1 day

3.

Agitation by rotary shaker, 180 rpm, 37 °C

15 hours

4.

Adhesion incubation in flask, 37 °C

1 to 3 hours

5.

Agitation by hand, 20 °C

2 to5 minutes

6.

Adhesion incubation in flask, 37 °C

1 to 3 hours

7.

Agitation by hand, 20 °C

2 to 5 minutes