A comparative review of cell culture systems for the study of microglial biology in Alzheimer’s disease

Table 2 Microglia properties by cell line

	Primary Microglia	BV2	N9	HMO6	EOC	HAPI
MAC-1	+	+	+	+	+	+
LPS stimulation	+	+	+	+	+	+
lL-1β release	+	-	+	-	N/A	+
TNF- release (Following LPS)	+	+	+	+	N/A	+
Phagocytosis	+	+	+	+	+	+
Peroxidase	-	-	-	-	-	-
Non-specific esterase	+	+	+	+	+	+
GFAP	-	-	-	-	-	-
GC	-	-	-	-	-	-
·NO production	+	+	+	-	+	+
Aβ induced lL-β	+	+	+	-	N/A	N/A
Aβ induced TNF-	+	+	+	+	N/A	N/A
Aβ phagocytosis	+	+	+	N/A	N/A	N/A
Number of articles in Pub Med^a	302	142	91	8	18	19

The table represents properties of individual cell lines as reported by citations in the PubMed Database (http://www.ncbi.nlm.nih.gov/). (+) Positive for trait, (−) negative for trait. ^aSpecific PubMed search term ‘primary microglial cell culture’ (from year 2000 to present; includes both studies of endogenous microglia as well as primary cultures of microglial isolates). Aβ, amyloid β; GC, galactocerebroside; GFAP, glial fibrillary acidic protein; LPS, lipopolysaccharide.

ISSN: 1742-2094