Figure 2
Dasatinib attenuated Aβ1-42 stimulated-microglial secretion of the proinflammatory cytokine, TNF-α. Primary microglia were unstimulated (control), DMSO vehicle treated (veh), or stimulated for 24 hours with 10 μM Aβf in the presence/absence of 100 nM dasatinib. (A) Media was collected from treated cells and used to quantify changes in TNF-α secretion via ELISA. Secreted values were averaged +/−SEM. (B) After media removal, the treated cells were used to assess cell viability via the MTT reduction assay. Absorbance values (560/650 nm) were averaged+/−SEM. (**P <0.01 from control, ***P <0.001 vs. vehicle, dasatinib and Aβ + dasatinib).