Figure 8

Dasatinib infusion attenuated microgliosis, protein phosphotyrosine and active phospho-Src immunoreactivity with no effect on GFAP immunoreactivity in APP/PS1 brains. Right hemispheres from brains of dasatinib infused, vehicle infused and APP/PS1 controls animals were fixed, serially sectioned and immunostained using (A) anti-phosphotyrosine (4 G10), anti-CD68, anti-GFAP, and anti-phospho-Src (pSrc) antibodies. Representative images from the CA1 region of the hippocampus (HP) as well as the temporal cortex (TC) are shown with select high magnification TC fields. (B) High magnification images of pSrc immunoreactivities from control APP/PS1 temporal cortex and hippocampus demonstrate robust plaque-associated immunoreactivity (arrow) along with vascular immunoreactivity (arrow). (C) Fluorescent double-labeling of control APP/PS1 brains demonstrated clear pSrc (red) immunoreactivity co-localizing with CD68 (green) staining. (D). Relative levels of pSrc immunoreactivity in the temporal cortex were quantified from serial stained sections from vehicle and dasatinib infused brains, averaged (+/−SEM), and graphed (***P <0.001 via student’s t-test).