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Table 2 MMP-9-digested MBP peptides in vitro

From: Immunodominant fragments of myelin basic protein initiate T cell-dependent pain

ASQKRPSQR10 HGSKY↓LATAS20 TMDHARHGFL30 PRH↓RDTGILD40 SIGRFFGGDR50 GAPKRGSGKD60 SHHPARTAHY70 GSLPQKSHGR80 TQDENPVVHF90 F↓KN↓IVTP↓RTP100 PP↓SQGKGRGL110 SLSRFSWGAE120 GQRPGFGYGG130 RASD↓YKSAHK140 GFKGVDAQGT150 LSK↓IFKLGGR160 DSRSGSPMAR170 R171

Peptide sequences

Molecular mass, Da

 
 

Calculated

Measured

A2SQKRPSQRHGSKYLATASTMDHARHGFLPRHRDTGILDSIGRFFGGD

14,482

14,498

RGAPKRGSGKDSHHPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTP

RTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASD134

L16ATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSH

10,229

10,247

HPARTAHYGSLPQKSHGRTQDENPVVHFFKNIVTPRTPPPSQGKGRG109

L16ATASTMDHARHGFLPRHRDTGILDSIGRFFGGDRGAPKRGSGKDSH

8,357

8,356

HPARTAHYGSLPQKSHGRTQDENPVVHFF91

S103QGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHKGFKGVDA

7,307

7,296

QGTLSKIFKLGGRDSRSGSPMARR171

R34DTGILDSIGRFFGGDRGAPKRGSGKDSHHPARTAHYGSLPQKSH

7,010

7,018

GRTQDENPVVHFFKNIVTP97

I94VTPRTPPPSQGKGRGLSLSRFSWGAEGQRPGFGYGGRASDYKSAHK

6,293

6,284

GFKGVDAQGTLSK153

  
  1. MMP-9 proteolysis of human MBP was performed as detailed in Figure 3, followed by MALTI-TOF MS analysis of the digest fragments. The proteolysis of MBP by MMP-9 was performed at a 1:1,000 enzyme-substrate ratio. The sequences of intact MBP and digest fragments are shown. The arrows indicate the MMP-9 cleavage sites. The dot indicates the initiating Met-1.