Figure 5

Involvement of c-Src/PDGFR/PI3K/Akt in MMP-9 mRNA expression induced by JEV in RBA-1 cells. (A) Cells were pretreated with AG1296 (AG12, 10 μM), PP1 (10 μM), or LY294002 (LY, 30 μM) for 1 h, and then infected with JEV for 6 h. RNA samples were analyzed by RT-PCR to determine levels of MMP-9 mRNA. (B) Cells were transiently transfected with an MMP-9-luc reporter gene, pretreated with AG1296 (AG12, 10 μM), PP1 (10 μM), or LY294002 (LY, 30 μM) for 1 h, and then infected with JEV for 6 h. The promoter activity of MMP-9 was measured. Data are expressed as mean ± S.E.M. for five independent experiments. ^#P < 0.01, as compared with the cells exposed to JEV alone.