Figure 2

Histopathology of spinal cord lesions in EAE-induced SRA ^−/− and WT mice. A) Representative images of LFB and hematoxylin staining of EAE-induced WT and SRA^−/− mice (n = 5). Although LFB staining of WT mice showed demyelination (Ai) in parallel to massive cell infiltration (hematoxylin staining) (Aii), LFB (Aiii) and hematoxylin (Aiv) staining of SRA^−/− mice showed reductions or complete loss of demyelination and infiltration. B) Representative images of immunohistology staining of the lumbar sections of EAE-induced WT and SRA^−/− mice (n = 5). DAPI (nuclear staining), CD3 (a T-cell marker) and CD11b (a microglia/macrophage marker). Original magnification: spinal cord - upper panels – 500 μm; lower panels – 100 μm.