Figure 4

In vitro effect of MK-591 on Aβ formation and amyloid-β precursor protein metabolism. N2A-APPswe cells were incubated with increasing concentration of MK-591 or vehicle for 24 h, and conditioned media and cell lysates collected. (A) Aβ1-40 levels in the supernatant assayed by sandwich ELISA (n = 4 per each condition; *P <0.01). (B) Representative western blots of APP, ADAM-10, BACE-1, PS1, nicastrin, APH-1, and Pen-2 in the lysates of MK-591 or vehicle-treated cells. (C) Densitometric analyses of the immunoreactivities to the antibodies shown in panel B (*P <0.01). ADAM-10: disintegrin and metalloproteinase domain-containing protein 10; APH-1: anterior pharynx-defective 1; APP: amyloid-β precursor protein; BACE-1: β-site amyloid precursor protein cleaving enzyme 1; ELISA: enzyme-linked immunosorbent assay; N2A-APPswe: neuro-2 A neuroblastoma cells expressing human APP carrying the K670N/M671L Swedish mutation; Pen-2: presenilin enhancer 2; PS1: presenilin1.