Figure 5

In vitro effect of MK-591 on cAMP response element-binding protein and Notch. N2A-APPswe cells were incubated with increasing concentration of MK-591 or vehicle for 24 hours, and cell lysates collected for immunoassays. (A) Representative western blots of total CREB, its phosphorylated form (p-CREB) at Ser133, and Sp1 levels. (B) Densitometric analyses of the immunoreactivities to the antibodies to CREB, p-CREB and Sp1 (*P <0.01). (C) Representative western blots of NICD in the lysates of cells treated with MK-591, L685,458 or vehicle groups. (D) Densitometric analyses of the immunoreactivities to the antibodies NICD (n = 4; *P <0.01). CREB: cAMP response element-binding protein; NICD: Notch intracellular domain; N2A-APPswe: neuro-2 A neuroblastoma cells expressing human APP carrying the K670N/M671L Swedish mutation.