Minocycline and naltrexone block microglial activation.
(A) Quantification of activated Iba1 + IR cells in cortex. Ethanol, poly I:C and ethanol-poly I:C treatment groups show increased microglial activation. Minocycline and naltrexone decreased ethanol-poly I:C-activated Iba1 + IR cells. (C, control; E, ethanol; P, poly I:C; EP, ethanol-poly I:C; EPM, ethanol-poly I:C-minocycline; EPN, ethanol-poly I:C-naltrexone.). (B) Representative images from vehicle control (C), ethanol-poly I:C (EP), ethanol-poly I:C-minocycline (EPM) and ethanol-poly I:C-naltrexone (EPN) groups in cortex. In control, EPM and EPN groups, most microglia are in a resting state: small cell bodies with thin, highly ramified processes. In the EP-treated group, microglia are activated: large cell bodies, irregular shape and intensified Iba1 staining. **P <0.01, compared with control group. ##
P <0.01, compared with poly I:C group. $$
P <0.01, compared with ethanol-poly I:C group. Scale bar, 200 μm.