Minocycline and naltrexone blunt ethanol-poly I:C-induced caspase-3 + IR. (A) Brain sections were stained with polyclonal cleave caspase-3 (Asp175) antibody. Immunolabeling was visualized by using nickel-enhanced 3,3′-diaminobenzidinne (DAB) as described in the methods. The number of caspase-3 + IR cells in cortex was significantly increased in ethanol, poly I:C and ethanol-poly I:C treatment groups. Minocycline and naltrexone reduced ethanol-poly I:C-induced caspase-3 expression. (C, control; E, ethanol; P, poly I:C; EP, ethanol-poly I:C; EPM, ethanol-poly I:C-minocycline; EPN, ethanol-poly I:C-naltrexone). (B) Images are representative of vehicle control (C), ethanol-poly I:C (EP), ethanol-poly I:C-minocycline (EPM) and ethanol-poly I:C-naltrexone (EPN) groups in cortex. Scale bar, 50 μm. *P <0.05, **P <0.01, compared with vehicle control. ##
P <0.01, compared with poly I:C. $$
P <0.01, compared with ethanol-poly I:C.