Figure 1

Characterization and kinetics of HIV-1 ^wt and HIV-1∆Vpr virus particles. (a) HEK 293 T cells were transfected with 5 μg of HIV-1 YU2^wt and HIV-1 YU2∆Vpr plasmids. Viruses collected after 72 hours were filtered, concentrated by ultracentrifugation and western blot analysis was performed for Gag and Vpr. (b) Productive infection of MDMs by viruses. MDMs were infected with HIV-1^wt and HIV-1∆Vpr viruses at an MOI of 0.1 or left untreated as negative control (NT). The release of virus particles from infected MDM culture was monitored by p24 ELISA using the supernatants on days as indicated. The data are representatives of one of six independent experiments.