Induction of neurotrophic factors in EAE mice treated with MOG-DNA. mRNA expression levels for each gene were determined in CNS tissue by RT-PCR relative quantification, as described in Methods. Gapdh was used as endogenous control. Graphs indicate fold changes in gene expression in MOG-DNA treated mice relative to plasmid control mice (calibrators). ΔCT values were compared by means of Student’s t tests. Statistically significant differences are shown with asterisks.