Promotion of remyelination in mice vaccinated with MOG-DNA. (A) Changes in mRNA expression levels for Pdgfa and Sema3f were determined in CNS from DNA-vaccinated mice on day 14 after EAE induction using RT-PCR relative quantification. A statistically significant increase was observed for Pdgfa (p = 0.017) in the group of animals vaccinated with MOG vs. pCi; for Sema3f, differences in expression did not reach statistical significance (p = 0.178). All values are normalized to Gapdh mRNA. (B) Expression of Olig2 by OPC was examined in spinal cords of MOG-DNA and plasmid control mice (brown staining). Quantification revealed that Olig2+-cells were increased in the CNS after treatment with DNA encoding MOG compared to control animals.