Figure 1

Release of neurotrophic and proinflammatory factors from PAR-2 activated rat microglia. Cells were treated with 10 μM 2-Furoyl-LIGRLO-NH2 in the presence or absence of PAR-2 shRNA. The concentration of BDNF, IL-1β, TNF-α, and NO in the supernatant were determined at 0.0, 0.5, 1.0, 2.0, 12.0, 24.0, and 72.0 h after 2-Furoyl-LIGRLO-NH2 application as described previously (A, B, C, D). All values represent means ± SE. *Significantly different from control group (P < 0.05). qRT-PCR and immunoblot analysis were also shown to verify the decreased expression of PAR-2 in the PAR-2 shRNA treated rat microglia (E, F).