Figure 2From: Transient early neurotrophin release and delayed inflammatory cytokine release by microglia in response to PAR-2 stimulation Stage-specific effect of MCM on nuclear DNA fragmentation in rat cortical neurons. Neurons were incubated in unstimulated MCM (control), MCM at 1 h after 10 μM 2-Furoyl-LIGRLO-NH2 stimulation (early phase) and MCM at 72 h after stimulation (late phase) for 48 h. Internucleosomal DNA fragmentation was determined by a fluorescent TUNEL assay. Percentage of apoptotic cells was calculated by counting in at least 6 randomly chosen subfields. Values are means ± SE. *Significantly different from control group, P < 0.05; # No significantly different from control group, P > 0.05.Back to article page