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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Transient early neurotrophin release and delayed inflammatory cytokine release by microglia in response to PAR-2 stimulation

Figure 4

Stage-specific effect of a PAR-1 agonist and antagonist on neurotrophic and proinflammatory responses in rat microglia activated by 2-Furoyl-LIGRLO-NH2. (A) Microglia was stimulated by 2-Furoyl-LIGRLO-NH2 (10 μM), 2-Furoyl-LIGRLO-NH2 plus SCH79797 (1 μM), or 2-Furoyl-LIGRLO-NH2 (10 μM) plus TFLLR (0.5 μM). The concentration of BDNF in MCM that was collected at 1 h after stimulation was determined by ELISA. Values are means ± SE. *Significantly different from 2-Furoyl-LIGRLO-NH2 treated group, P < 0.05. (B, C, D) Microglia was stimulated by 2-Furoyl-LIGRLO-NH2, then SCH79797 or TRLLR was added 24 h later (during the late phase of microglial activation). The concentration of IL-1β, TNF-α, and NO in MCM collected at 72 h after 2-Furoyl-LIGRLO-NH2 stimulation were determined as described. Values are means ± SE. *Significantly different from 2-Furoyl-LIGRLO-NH2 treated group: P < 0.05. (E) Cell viability was assessed by MTT assay, and the percentage of surviving cells compared to control cells. Values are means ± SE. # No significantly different from control group, P > 0.05.

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