Figure 5
From: MicroRNA-124 as a novel treatment for persistent hyperalgesia
MicroRNA-124 treatment influences the M1/M2 balance and abolishes the genotype differences after interleukin (IL)-1β injection. Mice received an intrathecal injection of 100 ng miR-124 the day before intraplantar injection of 1 ng IL-1β. At 15 hours after IL-1β injection, the spinal cord was collected, and frozen sections of lumbar spinal cord (L2 to L5) were stained for M1 and M2 phenotypic markers. M1 and M2 marker expressions were compared in wild-type (WT) versus LysM-G protein–coupled receptor kinase (GRK)2+/− mice, and expression was quantified in 18 dorsal and 18 lumbar spinal-cord sections per group from 4 mice per group. The level of expression in IL-1β-treated WT mice was set at 100%. Data are expressed as means ± SEM.