IFN-γ-induced increase in the mobility of MHC class II compartments in astrocytes depends on intermediate filaments

Table 2 Percentage of directional and non-directional vesicles before and after ATP treatment for control and IFN-γ-treated WT and GFAP ^-/- Vim ^-/- astrocytes and slopes of the lines fitted to the vesicle mobility data

Genotype	Stimulation	Control		IFN-γ
	Mobility	Slope (a ± s.e.m.)^a	Vesicle	Slope (a ± s.e.m.)^a	Vesicle
			(%)		(%)
WT	Spontaneous
	Directional	0.633 ± 0.038^c (152)^b	8.0	0.575 ± 0.020^c (426)	20.3
	Non-directional	0.262 ± 0.006 (1748)	92.0	0.219 ± 0.005 (1674)	79.7
	ATP
	Directional	0.483 ± 0.056^c (49)	5.2	0.597 ± 0.059^c (62)	5.9
	Non-directional	0.266 ± 0.009 (901)	94.8	0.231 ± 0.007 (988)	94.1
GFAP ^-/- Vim ^-/-	Spontaneous
	Directional	0.581 ± 0.068^c (75)	4.4	0.553 ± 0.030^c (92)	5.7
	Non-directional	0.324 ± 0.008 (1625)	95.6	0.329 ± 0.009 (1508)	94.3
	ATP
	Directional	0.616 ± 0.069^c (33)	3.9	0.781 ± 0.148^c (20)	2.5
	Non-directional	0.298 ± 0.010 (817)	96.1	0.279 ± 0.012 (780)	97.5

^aThe slope values (a) ± s.e.m were obtained from the linear function fitted to the mobility data in Figure 6.
^bValues inside brackets are numbers of vesicles. The slopes of regression lines for mobility data of directional and nondirectional vesicles differed in control and IFN-γ treated WT and GFAP ^-/- Vim ^-/- cells under spontaneous conditions and after ATP treatment.
^c P <0.001 vs. non-directional mobility.

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