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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Transforming growth factor β1-induced astrocyte migration is mediated in part by activating 5-lipoxygenase and cysteinyl leukotriene receptor 1

Figure 7

TGF-β1 induces 5-LOX expression and translocation, and increases the production of CysLTs in astrocytes. (A, B) RT-PCR and Western blotting results showing that 5-LOX mRNA (A) and protein expression (B) were increased in astrocytes after exposure to 10 ng/ml TGF-β1 for 24 h. Results are expressed as the ratios to β-actin (A) or GAPDH (B). C, control; T, TGF-β1. (C) Immunocytochemical examination reveals 5-LOX translocation to the nuclear envelope (arrows) in astrocytes after exposure to 10 ng/ml TGF-β1. Scale bar, 50 μm. (D) ELISA shows the production of CysLTs was significantly increased with a peak at 12 h after exposure to 10 ng/ml TGF-β1. Data are reported as mean ± S.E.M.; n = 4 (A and B) 8 (D); **P <0.01 compared with control.

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