Figure 8
Effect of TGF-β1 on expression of CysLT receptors in astrocytes. (A, B) RT-PCR and Western blotting results showing that the mRNA (A) and protein expression (B) of CysLT1R, but not CysLT2R, in astrocytes increased after exposure to 10 ng/ml TGF-β1 for 24 h. Data are reported as mean ± S.E.M.; n = 4; **P <0.01 compared with control. Results are expressed as the ratios to β-actin (A) or GAPDH (B). C, control; T, TGF-β1. (C) Double immunofluorescence staining showing that TGF-β1 increased the expression of CysLT1R in GFAP-positive astrocytes. Scale bar, 100 μm.