Figure 2From: Efficient isolation of live microglia with preserved phenotypes from adult mouse brain Yield and survival of isolated CD11b + cells. Brain tissues were enzymatically digested followed by centrifugation in (A) 30% Percoll or (B) 0.9 mol/l sucrose to remove myelin. After immunomagnetic separation of the CD11b+ cell population, cells were stained with Live/Dead fixable green stain, and the proportion of surviving cells was determined by flow cytometry.Back to article page