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Figure 5 | Journal of Neuroinflammation

Figure 5

From: The neuroprotective effects of milk fat globule-EGF factor 8 against oligomeric amyloid β toxicity

Figure 5

MFG-E8 exerts neuroprotective effects by activating the Nrf-2-HO-1 pathway in microglia. (A) Immunofluorescence images of Nrf2 (green) and nuclei (Hoechst; blue) in microglia treated with the indicated concentrations of MFG-E8. Scale bar = 10 μm. (B) After microglia were treated with the indicated concentrations of MFG-E8 for 24 h, HO-1 protein expression levels were measured by ELISA. Significant differences compared with untreated samples (*) are noted. ***: P <0.001 (one-way ANOVA with Dunnett’s post-hoc test). (C) Neuron − microglia co-cultures were pre-treated with MFG-E8 for 3 h in the presence of the HO-1 inhibitor SnMP. Then, the cultures were treated with oAβ for 24 h. The neuronal survival rate in the presence of MFG-E8 and SnMP was estimated. The results are presented as the means with S.E.M. (n = 3), in which 10 randomly selected fields were analyzed. Significant differences compared with oAβ plus MFG-E8 treated samples are noted. ***: P <0.001 (one-way ANOVA with Dunnett’s post-hoc test).

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