Plasminogen activator inhibitor type 1 (PAI-1) promoted microglial migration
. (A, B) Microglial cells around the protein injection sites were stained with anti-Iba-1 antibody at 48 hours after intrastriatal injection of vehicle (dialyzed elution buffer that was used for PAI-1 protein purification), denatured human PAI-1 wild-type, PAI-1 wild-type, or PAI-1 R346A mutant protein (1 μl; 1.5 μg/μl). Boxes indicate the 300 × 300 μm squares placed for cell counting. Immunohistochemistry results showed that Iba-1-positive cells were recruited into the injection site after injection of wild-type or R346 mutant PAI-1 protein. (B) Results are given as mean ± SD from three animals and six independent sections per animal. *P < 0.05, **P < 0.01, NS = not significant. (A) Representative images (scale bar, 300 μm). Asterisks indicate the injection sites (inj.). Guide cannula was stereotaxically located in the intrastriatal region (dotted line).