Constitutive activity of NF-kappa B in myeloid cells drives pathogenicity of monocytes and macrophages during autoimmune neuroinflammation

Table 2 Histological analyses.

	lysMCreIκBα^fl/fl	wild-type	p-value	n
Inflammatory Index [± SEM]	16.0 ± 1.9	6.8 ± 1.0	0.0003	5/5
CD 3 [cells/mm² ± SEM]	251 ± 19	102 ± 13	< 0.0001	5/5
Mac-3 [± SEM]	1123 ± 81	581 ± 100	< 0.0001	5/5
iNOS [± SEM]	333 ± 75	153 ± 19	0.0184	5/5
% Demyelination [± SEM]	8 ± 0.8	2 ± 0.6	< 0.0001	5/5
Neutrophils [± SEM]	339 ± 37	190 ± 25	n.s	5/5
Axonal densities [± SEM]	3.7 ± 0.5	2.9 ± 0.3	n.s	5/5

Blinded quantification of the inflammatory index after HE-staining, CD3 positive cells, Mac-3 positive macrophages/microglia, iNOS positive cells, demyelination after Luxol Fast Blue staining and axonal densities after silver impregnation were performed on day 15 p.i. marking the acute phase of the disease. Data are presented as cells/mm² for CD3, Mac-3, iNOS positive cells, percent demyelination for Luxol Fast Blue and relative axonal densities for silver impregnated axons. n.s = not significant.

ISSN: 1742-2094