Table 2 Summary of antibodies used in this study
From: Systemic immune challenges trigger and drive Alzheimer-like neuropathology in mice
Antibody | Manufacturer | Description/Nr | Dilution | Application |
|---|---|---|---|---|
β-Actin | Millipore, Billerica, MA, USA | Mouse monoclonal, clone 4, MAB1522 | 1:20,000 | IB |
Amyloid-β1–16 | Covance, Princeton, NJ, USA | Mouse monoclonal, clone 6E10, SIG-39320 | 1:1,000 | IHC/IB |
Amyloid-β1–40/42 | Millipore | Rabbit polyclonal, AB5076 | 1:2,000 | IHC |
APP (A4), N-term | Millipore | Mouse monoclonal, clone 22 C11, MAB348 | 1:2,000 | IHC/IB |
APP, C-term | Sigma-Aldrich Chemie GmbH, Buchs, CH | Rabbit polyclonal, A8717 | 1:4,000 | IB |
Cleaved Caspase-6 | Cell Signaling Technology, USA | Rabbit polyclonal, Nr. 9751 | 1:500 | IHC |
CD68 | AbD Serotec Ltd, Oxford, UK | Rat monoclonal, MCA341R | 1:2,000 | IHC |
GFAP | Dako Schweiz AG, Baar, CH | Rabbit polyclonal, Z334 | 1:5,000 | IHC |
pTauT205 | Abcam, Cambridge, UK | Rabbit polyclonal, ab4841 | 1:1,000 | IHC/IB |
PHF-Tau | Thermo Fisher Scientific, Fremont, CA, USA | Mouse monoclonal, MN1060 | 1:1,000 | IB |
Tau-Ab2 | Thermo Fisher Scientific | Mouse monoclonal, total Tau, clone Tau-5 | 1:4,000 | IB |