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Table 2 Summary of antibodies used in this study

From: Systemic immune challenges trigger and drive Alzheimer-like neuropathology in mice

Antibody Manufacturer Description/Nr Dilution Application
β-Actin Millipore, Billerica, MA, USA Mouse monoclonal, clone 4, MAB1522 1:20,000 IB
Amyloid-β1–16 Covance, Princeton, NJ, USA Mouse monoclonal, clone 6E10, SIG-39320 1:1,000 IHC/IB
Amyloid-β1–40/42 Millipore Rabbit polyclonal, AB5076 1:2,000 IHC
APP (A4), N-term Millipore Mouse monoclonal, clone 22 C11, MAB348 1:2,000 IHC/IB
APP, C-term Sigma-Aldrich Chemie GmbH, Buchs, CH Rabbit polyclonal, A8717 1:4,000 IB
Cleaved Caspase-6 Cell Signaling Technology, USA Rabbit polyclonal, Nr. 9751 1:500 IHC
CD68 AbD Serotec Ltd, Oxford, UK Rat monoclonal, MCA341R 1:2,000 IHC
GFAP Dako Schweiz AG, Baar, CH Rabbit polyclonal, Z334 1:5,000 IHC
pTauT205 Abcam, Cambridge, UK Rabbit polyclonal, ab4841 1:1,000 IHC/IB
PHF-Tau Thermo Fisher Scientific, Fremont, CA, USA Mouse monoclonal, MN1060 1:1,000 IB
Tau-Ab2 Thermo Fisher Scientific Mouse monoclonal, total Tau, clone Tau-5 1:4,000 IB
  1. APP, amyloid precursor protein; GFAP, glial fibrillary acidic protein; IB, immunoblotting; IHC, immunohistochemistry; pTau, phosphorylated Tau; PHF, paired helical filament.