Skip to main content
Figure 2 | Journal of Neuroinflammation

Figure 2

From: c-Src-dependent EGF receptor transactivation contributes to ET-1-induced COX-2 expression in brain microvascular endothelial cells

Figure 2

Transactivation of EGFR is involved in ET-1-induced COX-2 expression. (A,B) Cells were pretreated with various concentrations of AG1478 (0.1, 1, or 10 μM) for 1 h and incubated with 10 nM ET-1 for 6 h (A) or 1 h (B). (C) Time dependence of ET-1-induced EGFR phosphorylation; cells were treated with 10 nM ET-1 for the indicated time intervals in the presence or absence of AG1478 (10 μM). The phosphorylation of EGFR was analyzed by Western blot using an anti-phospho-EGFR antibody. (D) Cells were transfected with shRNA of EGFR (sh-EGFR) for 24 h (Topo: as a control) and incubated with 10 nM ET-1 for 6 h. The COX-2 protein (A,D) and mRNA (B) were analyzed by Western blotting and RT-PCR, respectively. Data are expressed as mean ± SEM of three individual experiments (n = 3). * P < 0.05, # P < 0.01 as compared with cells stimulated by ET-1 alone.

Back to article page