The enteric bacterial metabolite propionic acid alters brain and plasma phospholipid molecular species: further development of a rodent model of autism spectrum disorders

Table 5 Changes (percent composition) in rat brain phosphatidylinositol molecular species following intraventricular infusion with propionic acid (PPA) and phosphate buffered saline (PBS)

Molecular weight	Molecular species	Diacyl species	PBS	PPA
835	D34:1	16:0/18:1	0.53 ± 0.05	0.54 ± 0.07
859	D36:3	18:1/18:2	1.01 ± 0.14	0.79 ± 0.01*
857	D36:4	16:0/20:4	7.71 ± 0.32	7.02 ± 0.45
887	D38:3	18:0/20:3	9.07 ± 0.69	9.71 ± 0.73
885	D38:4	18:0/20:4	74.17 ± 1.35	74.60 ± 1.27
883	D38:5	18:1/20:4	5.53 ± 0.73	5.44 ± 0.41
881	D38:6	16:0/22:6	0.80 ± 0.04	0.79 ± 0.04
913	D40:4	18:0/22:4	0.41 ± 0.01	0.17 ± 0.03*
909	D40:6	18:0/22:6	0.77 ± 0.10	0.94 ± 0.12
Total%			100	100
∑ Monounsat			0.53 ± 0.05	0.54 ± 0.07
∑ Polyunsat			99.46 ± 0.05	99.46 ± 0.07

Values (nanomole percent by weight composition) represent means ± standard errors. Means in the same row accompanied by asterisks are significantly different between treatments at LSD = 0.05, n = 12 per treatment group. D represents diacyl species. Monounsat, monounsaturated fatty acids; PBS, phosphate buffered saline solution, polyunsat, polyunsaturated fatty acids; PPA, propionic acid. Phosphatidylinositol molecular species were identified using a precursor ion scan of m/z 241 in ESI negative mode. The lipid components in the table are arranged based on the molecular species composition.

ISSN: 1742-2094