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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Secreted phospholipase A2-IIA-induced a phenotype of activated microglia in BV-2 cells requires epidermal growth factor receptor transactivation and proHB-EGF shedding

Figure 2

sPLA 2 -IIA induces epidermal growth factor receptor (EGFR) transactivation in microglial cells. (A) EGFR expression on untreated BV-2 cells was analyzed by flow cytometry (open black curves) and compared with isotype controls (solid grey curves). (B) Flow cytometry analysis of phospho-EGFR levels: a) BV-2 cells were untreated (solid black curves) or treated with 1 μg/ml of sPLA2-IIA (solid dark grey curves) for different times; b) BV-2 cells were untreated (open black curves), treated with 1 μg/ml of sPLA2-IIA (open dark grey curves), or treated with 0.4 μM of EGF (open green curves) for 15 minutes, and compared with isotype controls (solid light grey curves). (C) Flow cytometry analysis of phospho-EGFR levels in primary microglial cells. Cells untreated (open black curves) and treated with 1 μg/ml of sPLA2-IIA for 15 (open dark grey curves). Solid light grey curves show negative controls in which the primary antibody was omitted. Results shown are representative of three independent experiments.

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