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Table 2 Primary antibodies

From: Clusters of activated microglia in normal-appearing white matter show signs of innate immune activation

Primary antibody

Dilution

Company

Proteolipid protein (PLP, clone plpc1)

1:500

Serotec Ltd.

HLA-DR (clone LN3)

1:75

eBioscience

CD 68 (clone KP1)

1:100

DAKO

gp91phox

1:100

[18]

p22phox

1:100

Santa Cruz

p47phox

1:50

Abcam

Amyloid precursor protein (APP)

1:2000

Millipore

CD3

1:100

DAKO

Glucose transporter-1

1:100

Abcam

FITC-labeled fibrinogen

1:100

DAKO

Claudin-5

1:100

Invitrogen

Glial fibrillary acidic protein (GFAP)

1:20

Monosan

Neurofilament (NF)

1:100

Abcam

Matrix metalloproteinase-2 (MMP2)

1:100

Abcam

Matrix metalloproteinase-9 (MMP9)

1:500

Calbiochem

Tumor necrosis factor-α (TNF-α)

1:200

Acris

Interleukin-4 (IL4)

1:70

R&D Systems

Interleukin-10 (IL10)

1:100

R&D Systems