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Figure 6 | Journal of Neuroinflammation

Figure 6

From: Post-ischemic estradiol treatment reduced glial response and triggers distinct cortical and hippocampal signaling in a rat model of cerebral ischemia

Figure 6

The decrease in pAkt Ser-473 levels induced by ischemia and its subsequent rescue by estradiol treatment primarily occurs in neurons within the ischemic area. Representative coronal brain sections corresponding to bregma −1.08 of control (SV) and pMCAO (IV) rats, and from estradiol-treated pMCAO animals (IE group). Images show the ipsilateral ischemic cortex double stained for GFAP (red) and pAkt Ser-473 (green). pMCAO (IV group) resulted in a decrease in the intensity of pAktSer-473 staining and an increase in GFAP staining intensity 54 h after the induction of ischemia, when compared with the control (IV) group. Post-pMCAO estradiol treatment rescued this decrease in pAkt Ser-473 levels and decreased GFAP staining, resulting in levels similar to those of the control (SV) group. The pAkt Ser-473 immunoreactivity (green) did not co-localize with that of GFAP (red). Representative cells exhibiting pAkt Ser-473-positive cytoplasmic labeling are indicated in SV and IE panels (arrows). The size and morphology of these cells strongly suggests they are neurons. Scale bar = 25 μm.

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