Table 1 Antibodies used for immunostaining or immunoblotting
From: Inflammation modulates expression of laminin in the central nervous system following ischemic injury
Antigen | Antibody | Dilution | Source | Catalog no. | Method |
|---|---|---|---|---|---|
NeuN | Mouse monoclonal | 1:1,000 | Chemicon | MAB377 | IHC |
Iba-1 | Rabbit polyclonal | 1:1,000 | Wako | 019-19741 | IHC |
TLR9 | Rabbit polyclonal | 1:500 | Imgenex | IMG431 | WB |
CD14 | Goat polyclonal | 1:1,000 | Santa Cruz Biotechnology | sc-6999 | WB |
Fibrin | Mouse monoclonal | 1:200 | A gift of Dr Galanakis | [45] | WB |
Collagen IV | Rabbit polyclonal | 1:500 | Chemicon | AB756 | WB |
Pan-laminin | Rabbit polyclonal | 1:1,000 | Sigma-Aldrich | L9393 | IHC |
| Â | Â | 1:5,000 | Â | Â | WB |
α-Tubulin | Mouse monoclonal | 1:7,000 | Upstate Biotechnology | 05-829 | WB |
Occludin | Mouse monoclonal | 1:250 | Zymed | 33-1500 | IHC |
HIF-1α | Rabbit polyclonal | 1:1,000 | Bethyl Laboratories | A300-286A | WB |
COX-2 | Mouse monoclonal | 1:500 | BD Biosciences | 610203 | WB |
Mac-2 | Rat monoclonal | 1:1,000 | Cedarlane | CL8942AP | WB |
CD45 | Rat monoclonal | 1:500 | BD Biosciences | 553076 | IHC |
VEGF | Rabbit polyclonal | 1:200 | Thermo scientific | RB-9031 | WB |
GFAP | Rabbit polyclonal | 1:1,000 | DAKO | Z0334 | IHC |