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Figure 6 | Journal of Neuroinflammation

Figure 6

From: (−)-Epigallocatechin gallate inhibits endotoxin-induced expression of inflammatory cytokines in human cerebral microvascular endothelial cells

Figure 6

Effect of (−)-epigallocatechin gallate on the permeability of human cerebral microvascular endothelial cells/D3. (A) Human cerebral microvascular endothelial cells/D3 (hCMEC/D3) were cultured in 0.4-μm Millicell hanging cell culture inserts to become confluent and then treated with lipopolysaccharide (LPS) (100 ng/mL) in the presence or absence of (−)-epigallocatechin gallate (EGCG) at the indicated doses for 24 h. After treatment, fluorescein sodium salt was loaded to the apical filter compartment (1 μM) and the fluorescence in the basolateral compartment was measured within 1 h. (*P < 0.05, **P < 0.01). (B) hCMEC/D3 at 1 × 105 per well were plated on collagen type I-coated 96W10E + electrode arrays (Applied Biophysics). The cells were then allowed to form monolayers reaching stable transendothelial electrical resistance (TEER) values. After 4 days (with a media change every 2 days), the monolayers were exposed to various concentrations of LPS with or without EGCG pretreatment at the indicated concentrations. The readings were acquired by the 1600R ECIS system continuously for 48 h at \00 Hz and at 30-minute intervals. Representative data from three independent experiments are shown.

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