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Table 1 Primer sequences for the quantitative real time PCR

From: (−)-Epigallocatechin gallate inhibits endotoxin-induced expression of inflammatory cytokines in human cerebral microvascular endothelial cells

Gene name

Forward

Reverse

TNF-α

5’-CGA GTG ACA AGC CTG TAG C-3’

5’-GGT GTG GGT GAG GAG CAC AT-3’

IL-1β

5’-AAG CTG ATG GCC CTA AAC AG-3’

5’-AGG TGC ATC GTG CAC ATA AG-3’

MCP-1/CCL2

5’-CAT AGC AGC CAC CTT CAT TCC-3’

5’-TCT GCA CTG AGA TCT TCC TAT TGG-3’

ICAM-1

5’-CCT TCC TCA CCG TGT ACT GG-3’

5’-AGC GTA GGG TAA GGT TCT TGC-3’

VCAM-1

5’-GGG AGC TCT GTC ACT GTA AG-3’

5’-ATC CGT ATC CTC CAA AAA CT-3’

Occludin

5’-AAG CAA GTG AAG GGA TCT GC-3’

5’-GGG GTT ATG GTC CAA AGT CA-3’

Claudin 5

5’-GTC TTT ACT CCA TCG GCA GG-3’

5’-TTT TTT TTT TTT GAG AGT TCA AAC C-3’

TLR4

5’-CAT TGC TTC TTG CTA AAT GCT G-3’

5’-GGA TTA AAG CTC AGG TCC AGG-3’

MyD88

5’-CCG CGC TGG CGG AGG AGA TGG AC-3’

5’-GCA GAT GAA GGC ATC GAA ACG CTC-3’

GAPDH

5’-GGT GGT CTC CTC TGA CTT CAA CA-3’

5’-GTT GCT GTA GCC AAA TTC GTT GT-3’

  1. GAPDH, glyceraldehyde 3-phosphate dehydrogenase; ICAM, intercellular adhesion molecule; MCP, monocyte chemotactic protein; TLR, Toll-like receptor; VCAM, vascular adhesion molecule.