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Figure 10 | Journal of Neuroinflammation

Figure 10

From: Time-dependent effects of hypothermia on microglial activation and migration

Figure 10

Protective effect of hypothermia against microglial neurotoxicity. Microglia/neuroblastoma co-culture scheme (A, B). BV-2 microglial cells were seeded in triplicate at a density of 1.5 x 104 cells per well in a 96-well plate. B35 neuroblastoma cells (3.75 x 104 cells per well) stably expressing EGFP were added onto the BV-2 microglial cells three hours prior to LPS (100 ng/ml) treatment. The co-culture of microglia and neuroblastomas was performed under normothermic or hypothermic conditions as indicated. At the end of the co-culturing, the EGFP-positive cells were counted under a fluorescence microscope to evaluate B35 neuroblastoma cell survival (C). **P < 0.01; different from the LPS-treated group under normothermia. LPS alone did not affect either B35 or BV-2 cell viability (E). Results are the mean ± SD (n = 3). Representative microscopic images are shown (D). Scale bar = 200 μm. EGFP, enhanced GFP; LPS, lipopolysaccharide; n, number; O/N, overnight.

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