Skip to main content
Figure 11 | Journal of Neuroinflammation

Figure 11

From: Time-dependent effects of hypothermia on microglial activation and migration

Figure 11

Protective effect of hypothermia against microglial neurotoxicity: co-cultures of primary microglia and neuroblastoma cells. B35-EGFP neuroblastoma cells were added onto BV-2 microglial cells or primary microglia cultures three hours prior to LPS (100 ng/ml) treatment. The co-culture of microglia and neuroblastoma was performed under normothermia (37 °C) or hypothermia (33 °C, 29 °C) as indicated (A, B). At the end of the co-culture, the EGFP-positive cells were counted under fluorescence microscope to evaluate B35 neuroblastoma cell survival (C). Results are mean ± SD (n = 3). *P < 0.05, **P < 0.01; different from LPS-treated group under normothermia. LPS alone did not affect B35 cell viability (data not shown). Representative microscopic images are also shown (D). Scale bar = 200 μm. EGFP, enhanced GFP; LPS, lipopolysaccharide; n, number; O/N, overnight

Back to article page