Figure 13

Protective effect of hypothermia against neurotoxicity of microglia-conditioned media. Microglia-conditioned media (MCM) were collected after stimulation of primary microglia cultures with LPS under normothermic or hypothermic condition as indicated (A, B). MCM were added to primary cortical neurons and the viability was assessed by a MTT assay 24 hours later (C). Results are mean ± SD (n = 3). *P < 0.05; different from LPS-treated group under normothermia. LPS, lipopolysaccharide; MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; n, number; O/N, overnight.