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Figure 14 | Journal of Neuroinflammation

Figure 14

From: Time-dependent effects of hypothermia on microglial activation and migration

Figure 14

Neuroprotective effect of hypothermia against oxidative or nitrosative stress. B35-EGFP neuroblastoma cells were seeded in triplicate at a density of 5 x 104 cells per well in 96-well plate, and then treated with H2O2 (0.5 mM) or SNP (0.1 mM) under normothermic (37 °C) or hypothermic (33 °C or 29 °C) conditions for 24 hours. After incubation B35-EGFP cells were counted (A, lower) or subjected to a MTT assay (B) for assessment of cell viability. Representative images of the fluorescence microscopy are shown (A, upper). Scale bar = 200 μm. Results are the mean ± SD (n = 3). *P < 0.05; significantly different from H2O2- or SNP-treated group under normothermia. EGFP, enhanced GFP; LPS, lipopolysaccharide; MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; n, number; SNP, sodium nitroprusside.

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