Figure 18

Effects of local hypothermia on microglial proliferation in vivo . A focal needle injury and BrdU injection (i.p., 200 mg/kg) were done under normothermic or hypothermic condition as indicated (A). Iba-1 (red) or BrdU (green) immunochemistry was done to determine the effects of local hypothermia on microglial proliferation in vivo (B). Asterisks indicate the injury site. Arrows indicate Iba-1 and BrdU double positive cells in the merged images. A representative microscopic image for each condition is shown (magnification, x200 or x400). Scale bar = 200 μm. BrdU, 5-bromo-2’-deoxyuridine; i.p., intraperitoneally.