Skip to main content
Figure 2 | Journal of Neuroinflammation

Figure 2

From: Time-dependent effects of hypothermia on microglial activation and migration

Figure 2

Time-dependent effects of hypothermia on NO production in LPS-stimulated microglial cells. Hypothermia was initiated at the different time points before or after LPS stimulation as shown in the experimental scheme (A). BV-2 microglial cells (5 x 104 cells/well in a 96-well plate) were incubated with LPS (100 ng/ml) under hypothermic (33 °C, 29 °C) or normothermic (37 °C) conditions as indicated. The amount of nitrite in the culture media was measured with the Griess reagent at 24 hours after LPS stimulation (B). Cell viability was examined by MTT assays. The results represent the percentage of surviving cells compared to the control (C). Results are the mean ± SD (n = 3). *P < 0.05, **P < 0.01; significantly different from the LPS-treated group under normothermia (37 °C). LPS, lipopolysaccharide; MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; n, number.

Back to article page