Figure 3

Effects of delayed hypothermia on NO production in LPS-stimulated microglial cells. Hypothermia was initiated at the different time points with the same duration as indicated in the experimental scheme (A). BV-2 microglial cells (5 x 10⁴ cells per well in a 96-well plate) were treated with LPS (100 ng/ml) for 24 hours under normothermic (37 °C) or hypothermic (29 °C) conditions as indicated (conditions 1 to 7). The concentration of nitrite in the culture media was measured with the Griess reagent at the end of the incubation (B). Cell viability was examined by MTT assays and the results were expressed as the percentage of surviving cells over the controls (C). Results are the mean ± SD (n = 3). *P < 0.05, **P < 0.01; significantly different from LPS treatment under normothermia. LPS, lipopolysaccharide; MTT, 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide; n, number.