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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Time-dependent effects of hypothermia on microglial activation and migration

Figure 5

Effect of hypothermia on pro-inflammatory gene expression in LPS-stimulated astrocyte cultures. Primary astrocyte cultures (5 x 105 cells per well in six-well plates) were treated with LPS (100 ng/ml) under hypothermia (29 °C) or normothermia (37 °C) as indicated (A). Total RNA was isolated at six hours after the LPS treatment. The levels of cytokine mRNA were determined by real-time RT-PCR. The data were normalized to GAPDH (B). Results are mean ± SD (n = 3). *P < 0.05, **P < 0.01; significantly different from the LPS-treated group under normothermia. LPS, lipopolysaccharide; n, number.

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