Figure 6

Effect of hypothermia on pro-inflammatory gene levels in LPS-stimulated microglial cells: conventional RT-PCR analysis. Primary microglial cells (5 x 10⁵ per well in six-well plates) were treated with LPS (100 ng/ml) under hypothermia (29 °C) or normothermia (37 °C) as indicated in the main text. Total RNA was isolated at six hours after the LPS treatment. The levels of cytokine and iNOS mRNA were determined by conventional RT-PCR (A). The data were normalized to GAPDH (B, C, and D). Results are mean ± SD (n = 3). *P < 0.05, **P <0.01; different from LPS-treated group under normothermia. LPS, lipopolysaccharide; n, number.