Table 1 DNA sequences of the primers used for RT-PCR.
From: Time-dependent effects of hypothermia on microglial activation and migration
cDNAs | RT-PCR methods | Primer sequences | GenBank accession No. | RT-PCR product size (bp) |
|---|---|---|---|---|
Mouse | Traditional | Forward, 5’-GCA ACT GTT CCT GAA CTC-3’ | NM_008361 | 382 |
il-1β |  | Reverse, 5’-CTC GGA GCC TGT AGT GCA-3’ |  |  |
Mouse | Real-Time | Forward, 5’-AGT TGC CTT CTT GGG ACT GA-3’ | NM_008361 | 102 |
il-1β |  | Reverse, 5’-TCC ACG ATT TCC CAG AGA AC-3’ |  |  |
Mouse | Traditional | Forward, 5’-CCC TTC CGA AGT TTC TGG CAG CAG C-3’ | NM_010927 | 497 |
inos |  | Reverse, 5’-GGC TGT CAG AGC CTC GTG GCT TTG G-3’ |  |  |
Mouse | Real-Time | Forward, 5’-GCC ACC AAC AAT GGC AAC A-3’ | NM_010927 | 103 |
inos |  | Reverse, 5’-CGT ACC GGA TGA GCT GTG AAT T-3’ |  |  |
Mouse | Traditional | Forward, 5’-CAT CTT CTC AAA ATT CGA GTG ACA A-3’ | NM_013693 | 411 |
tnf-α |  | Reverse, 5’-ACT TGG GCA GAT TGA CCT CAG-3’ |  |  |
Mouse | Real-Time | Forward, 5’-ATG GCC TCC CTC TCA GTT C-3’ | NM_013693 | 104 |
tnf-α |  | Reverse, 5’-TTG GTG GTT TGC TAC GAC GTG-3’ |  |  |
Mouse | Real-Time | Forward, 5’- GCC CTC TCT CTC CTC TTG CT -3’ | NM_013652 | 196 |
ccl4 |  | Reverse, 5’- GTC TGC CTC TTT TGG TCA GG -3’ |  |  |
Mouse | Real-Time | Forward, 5’- CGA CTG TTG CCT CTC GTA CA -3’ | NM_016960 | 177 |
ccl20 |  | Reverse, 5’- AGG AGG TTC ACA GCC CTT TT -3’ |  |  |
Mouse | Real-Time | Forward, 5’-AAG TGC TGC CGT CAT TTT CT-3’ | NM_021274 | 186 |
cxcl10 |  | Reverse, 5’-GTG GCA ATG ATC TCA ACA CG-3’ |  |  |
Mouse | Real-Time | Forward, 5’-AGT TGC CTT CTT GGG ACT GA-3’ | NM_031168 | 159 |
il-6 |  | Reverse, 5’-TCC ACG ATT TCC CAG AGA AC-3’ |  |  |
Mouse | Real-Time | Forward, 5’- AAG GAC CAG CTG GAC AAC AT -3’ | NM_010548 | 230 |
il-10 |  | Reverse, 5’- TTT TCA CAG GGG AGA AAT CG 3’ |  |  |
Mouse | Real-Time | Forward, 5’- CAT CGA TGA GCT GAT GCA GT -3’ | M86672 | 163 |
il-12 |  | Reverse, 5’- CAG ATA GCC CAT CAC CCT GT -3’ |  |  |
Rat/mouse | Traditional | Forward, 5’-ACC ACA GTC CAT GCC ATC AC-3’ | NM_008084 | 452 |
gapdh |  | Reverse, 5’-TCC ACC ACC CTG TTG CTG TA-3’ |  |  |
Mouse | Real-Time | Forward, 5’- TGG GCT ACA CTG AGC ACC AG -3’ | NM_008084 | 171 |
gapdh |  | Reverse, 5’- GGG TGT CGC TGT TGA AGT CA-3’ |  |  |
Rat | Traditional | Forward, 5’-CTT TCA TCA CAC AGG ACA GG-3’ | NM_031512.2 | 228 |
il-1β |  | Reverse, 5’-GTG ATG TTC CCA TTA GAC AGC-3’ |  |  |
Rat | Traditional | Forward, 5’-GAC GCC CCG GCC TTC CAA ATA AAT-3’ | NM_012675.3 | 800 |
tnf-α |  | Reverse, 5’-GCT GCC CCG ACT ATG TGC TCC TCA-3’ |  |  |
Rat | Traditional | Forward, 5’-CAG ACA GTT TCT GGT CGA TGT CAT GA -3’ | D44591 | 230 |
iNOS |  | Reverse, 5’-CTG CAT GGA ACA GTA TAA GGC AAA C -3’ |  |  |