Figure 3

CD200R1 expression in glial cultures. (A) CD200R1 mRNA was detected in primary microglial cultures but not in astroglia-enriched cultures using qRT-PCR. A clear decrease in CD200R1 mRNA expression was detected in microglial cultures six hours after LPS treatment. Bars are means + SEM of three cultures. ***P <0.001 versus C. Two-way ANOVA and Bonferroni post-test. (B) Histogram plots showing CD200R1 staining in mixed glial cultures by flow cytometry. A decrease was observed in cultures treated with LPS for 24 hours (red line) when compared to control cultures (green line). The black line shows control isotype staining. The results are representative of three cultures. ANOVA, analysis of variance; C, control; LPS, lipopolysaccharide; qRT-PCR, quantitative reverse transcriptase polymerase chain reaction; SEM, standard error of the mean.