Figure 4

CD200R1 expression in the absence of C/EBPβ. (A) Western blot showing C/EBPβ protein expression in total protein extracts of primary mixed glial cultures from control and LPS-treated wild-type and C/EBPβ-deficient mice. (B) CD200R1 mRNA expression in primary mixed glial cultures from control and LPS-treated (six hours) wild-type and C/EBPβ-deficient mice. The decrease in CD200R1 mRNA expression induced by LPS in wild-type cultures was not observed in C/EBPβ-deficient cultures. Bars are means + SEM of three to four independent experiments. ***P <0.001 versus C; ##P <0.01 versus wild type LPS-treated cultures. Two-way ANOVA and Bonferroni post-test. ANOVA, analysis of variance; C, control; C/EBPβ, CCAAT/enhancer binding protein β; LPS, lipopolysaccharide; SEM, standard error of the mean.