Figure 5
From: Inhibition of CD200R1 expression by C/EBP beta in reactive microglial cells
CD200R1 and C/EBPβ expression in BV2 cells. (A) CD200R1 immunostaining in control and LPS-treated BV2 cells. A clear decrease was observed 24 hours after LPS treatment. Bar = 50 μm. (B)C/EBPβ mRNA and (C) western blot showing C/EBPβ protein expression in control and LPS-treated (six hours) BV2-pCDNA and BV2-LAP cell clones. Notice the strong increase in C/EBPβ expression in BV2-LAP cells when compared to BV2-pCDNA cells. Bars are means + SEM of three independent experiments. ***P <0.001 versus C and ###P < 0.001 versus the equivalent experimental condition in BV2-pCDNA cells; two-way ANOVA and Bonferroni post-test. &P <0.05 and &&&P <0.001 versus C; Student’s t-test. ANOVA, analysis of variance; C, control; C/EBPβ, CCAAT/enhancer binding protein β; LPS, lipopolysaccharide; SEM, standard error of the mean.